TABLE 2.

Microscopic rate constants for phosphorylation of Tide by His₆-S6K1(ΔAID)-T389E

The reaction steps and rate constants refer to the Ordered Bi Bi system depicted in Scheme 1.

Reaction step	Rate constant	Value
E + ATP → E·ATP	k+1 (m-1 s-1)	(3.8 ± 0.2) × 104
E·ATP → E + ATP	k-1 (s-1)	0.22
E·ATP + Tide → E·ATP-Tide	k+2 (m-1 s-1)	≥106
E·ATP-Tide → E·ATP + Tide	k-2 (s-1)	≥190
E·ATP-Tide → E·ADP-pTide	k+3 (s-1)	8.6 ± 1.0
E·ADP-pTide → E·ADP + pTide	k+4 (s-1)	≥180
E·ADP + pTide → E·ADP-pTide	k-4 (m-1 s-1)	≥106
E·ADP → E + ADP	k+5 (s-1)	0.23 ± 0.01
E + ADP → E·ADP	k-5 (m-1 s-1)	(4.0 ± 0.2) × 104