Fig. 3.

Effect of PRP treatment on proteoglycan (PG) synthesis by NP and AF cells cultured in alginate beads (From: Akeda K et al. (2006) Spine, 31:959–966, with permission). During the last 4 h of the 72-h treatment period, the cells were radiolabeled with ³⁵S-sulfate (20 μCi/ml) and the amount of radiolabeled ³⁵S-PGs in the cell-associated matrix (CM) extract, the further-removed matrix (FRM) fraction and the media were quantified. PG synthesis in the PRP-treated NP and AF cells was significantly higher than that in the platelet-poor plasma (PPP)- and FBS-treated cells. SFM: serum free media