Figure 1.

HIV-specific activity of a polyclonal CD3⁺p58.2⁺ population. The lymphocyte population examined was derived from PBMCs of an HIV-infected patient. The freshly derived lymphocyte population contained 12% CD3⁺ cells expressing p58.2. Unfractionated cells or cells that had been depleted of CD4⁺, CD16⁺, and CD19⁺ cells were stimulated with phytohemagglutinin and cultured in the presence of 100 units/ml r-interleukin 2 for 20 days. HIV-specific CTL activity was analyzed at an E/T ratio of 25:1 against autologous B-LCLs infected with recombinant vaccinia viruses expressing HIV env, gag/pol, and nef proteins (17). CTL activity in the absence (hatched bar) or in the presence (black bar) of the anti-p58.2 Y249 mAb (IgM). Open bar indicates lysis of control target cells expressing the lacZ gene product. (Inset) The majority of cultured cells, in the CD8⁺ cell fraction, coexpressed CD3 and p58.2 antigens.