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Flow cytometric analysis. Spleen cells from wild-type, Fyn−/−, Lyn−/−, or Fyn/Lyn−/− mice were stained with (A) biotinylated mAb CS/2 or (B) anti-IgM mAb followed by avidin-phycoerythrin and fluorescein isothiocyanate-labeled RA3–6B2.