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. 1997 Sep 16;94(19):10324–10329. doi: 10.1073/pnas.94.19.10324

Figure 3.

Figure 3

(A) RT-PCR of RNA from 16.3 and 16.3ΔP cells. Lane M shows the molecular weight mass in base pairs. Lane 16.3 is the RT-PCR product obtained from 16.3 cells. Lane ΔP shows two RT-PCR products obtained from 16.3ΔP cells. (B) Expression of polβ protein of 16.3 and 16.3ΔP cell extracts analyzed by Western blot. Nuclear proteins (20 μg) were separated and immunoblotted as described in Figs. 1 and 2. Lane 16.3 represents the expression of WT polβ protein in 16.3 cell extracts. Lane ΔP shows the expression of both WT and truncated proteins in 16.3ΔP cell extracts. (C) BER activity of 50 μg protein of the 16.3 nuclear extracts is shown in lane 16.3. Lane ΔP shows that BER activity is absent in the presence of 50 μg of 16.3ΔP nuclear extracts. Lane M contains the molecular size markers in base pairs.