Figure 5.

Gel mobility shift assay of the 16.3 and 16.3ΔP nuclear extracts. A 51-bp double-stranded DNA was used as described in Materials and Methods. Lane 1 shows the free probe alone. Lane 2 represents 10 μg protein of 16.3 nuclear extracts. Lane 3 shows effects of cold oligonucleotide on 16.3 mobility. Lane 4 shows a supershift of 16.3 nuclear protein in the presence of polβ antibody. Lane 5 shows results for 16.3ΔP cells. Lane 6 shows activity is undetectable in 16.3ΔP nuclear extracts in the presence of oligonucleotides. Lanes 7–10 represent binding activity of 16.3 nuclear extracts in the presence of 1–10 μg nuclear 16.3ΔP protein.