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Western blot analysis of fractions obtained from dsDNA cellulose chromatography. Procedures related to loading of the column and elution are described in Materials and Methods. Lane 1 represents a 39-kDa WT polβ protein in flow-through and wash fractions. Lane 2 shows the same protein in the eluent. Proteins in flow-through and wash fractions of 16.3ΔP extract are shown in lane 3. Lane 4 shows a protein in eluents of 16.3ΔP extract.
