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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Gastroenterology. 2008 Jan 11;134(4):1007–1016. doi: 10.1053/j.gastro.2008.01.013

Figure 2. Klf5 is induced in response to infection with C. rodentium.

Figure 2

Frozen tissue sections from distal colon were isolated from mice given PBS (uninfected) or infected with C. rodentium and sacrificed 8 or 14 days post-infection (p.i.). (A) Tissues were stained with H& E, or for immunofluorescence using antibodies derived against Klf5 (green) or Ki67 (red). (B) The numbers of epithelial cells per crypt in colons of uninfected or infected mice were measured on the H&E sections. Five crypts per sample were counted. (C) Relative immunofluorescence intensities for Klf5 were determined on a per cell basis by quantitative analysis of Klf5 immunofluorescence images. For each condition, at least 200 cells positive for Klf5 expression were quantified for average fluorescence intensities.