Figure 3.

Ovarian steroids suppress MMP-1 production in rhIL-1α- stimulated fibroblasts. Confluent endometrial fibroblasts were cultured either without (lanes 1 and 2) or with (lanes 3 and 4) 1 ng/ml rhIL-1α, and in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of 1 nM 17β-estradiol and 100 nM progesterone. Collagenase activity was measured in culture supernatants after 24 h. Values are representative of four experiments on cells from different patients and are means ± SEM (n = 3).