Figure 2.

Western blot analysis of the PMCA pump protein. Erythrocyte IOV proteins were prepared and analyzed by immunoblot from healthy volunteers (lane 1), from the father (lane 2), from the propositus (lane 3), and from the neurologically symptom-free brother (lane 4). In lanes marked with (a) 15 μg protein and in those marked with (b) 7.5 μg protein was analyzed. In the case of the propositus, an extra lane with 3.75 μg protein (c) was also prepared. The quantitative evaluation of the Western blot is presented in B. Data represent the results of one experiment from four independent IOV preparations and Western blot analysis. The average enhancement of PMCA pump protein of the erythrocytes from the propositus was 2.9 ± 0.6 as compared with the control.