Figure 4.
Filled and intact preintegration site sequences for individual nonautonomous piggyBac1_ML elements from M. lucifugus and M. austroriparius, respectively. Target site duplications for each insertion are shaded. (A) Locus 340_3005. (B) Locus primer sequences (5′–3′) for the two loci presented are as follows: Mluc_cont1.015340_3005_L—TTGTACCAAAAGGTGCCAAA, Mluc_cont1.015340_3005_R—TTTCCTCATATACCATCCCATTTT; Mluc_cont1.000939_4755_L—CAAAATAAGGGAGAAAGGAAACA, Mluc_cont1.000939_4755_R—GGGCTGAGAAACAAGATCCA. (Mluc) M. lucifugus; (Maus) M. austroriparius.