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. 1997 Sep 16;94(19):10385–10390. doi: 10.1073/pnas.94.19.10385

Figure 3.

Figure 3

Infections with (a) YPIIIpIB1, (b) YPIIIp506, (c) YPIIIp503, or (d) YPIIIpIB71 for 8 h. TUNEL reaction was used to label 3′-OH termini with fluorescein. Anti-Y. pseudotuberculosis primary antibody and anti-rabbit 7-amino-4-methylcoumarin-3-acetic acid (AMCA) secondary antibody were used to label bacteria and rhodamine phalloidin was used to label actin filaments. Images of epifluorescence were scanned into Adobe photoshop and aligned to make a composite. The arrow indicates an infected macrophage positive for TUNEL reaction.