Table 1. Data-collection and processing statistics of the human tRNAGly acceptor-stem microhelix.
Values in parentheses are for the highest resolution shell.
| Beamline | DESY/HASYLAB X13 |
| Wavelength (Å) | 0.8148 |
| Space group | C2 |
| Unit-cell parameters (Å, °) | a = 37.12, b = 37.49, c = 30.38, β = 113.02 |
| Matthews coefficient VM (Å3 Da−1) | 2.33 |
| RNA duplexes per asymmetric unit | 1 |
| Solvent content† (%) | 59.4 |
| Measured reflections | 83227 |
| Unique reflections | 11547 |
| Resolution range (Å) | 50.0–1.20 (1.22–1.20) |
| Completeness (%) | 95.3 (93.7) |
| Multiplicity (%) | 7.2 (7.6) |
| Rmerge‡ (%) | 5.4 (24.9) |
| 〈I〉/〈σ(I)〉 | 18.1 (1.46) |
Estimated using the average partial specific volume calculated for RNA by Voss & Gerstein (2005 ▶).
R
merge =
, where I
i(hkl) and 〈I(hkl)〉 are the observed individual and mean intensities of a reflection with indices hkl, respectively,
is the sum over the individual measurements of a reflection with indices hkl and
is the sum over all reflections.