Table 1.
Sterol accumulation profiles of wild-type and ergosterol-deficient strains
| Yeast genotype | Sterols accumulated |
|---|---|
| WA1 (ERG11 ERG25 SLU1) | Ergosterol (65), zymosterol (6), fecosterol (9), episterol (9), lanosterol (5), 4,4-dimethylzymosterol (3) |
| erg25(*) | 4,4-Dimethylzymosterol (100) |
| erg11(*) | Lanosterol (86), 14-methylfecosterol (7), obtusifoliol (7) |
| erg11 erg25(*) | Lanosterol (94), 24-methylenelanosterol (6) |
| slu1 | Ergosta 5,7-dien-3β-ol (65), ergosterol (18), lanosterol (8), zymosterol (5), 14-methylfecosterol (3), fecosterol (1) |
| slu2 | Lanosterol (45), fecosterol (or ergosta-5,7-dien-3β-ol) (32), obtusifoliol (5), 14-methylfecosterol (15), ergosterol (3) |
| erg25 slu1(*) | Lanosterol (22), 4,4-dimethylzymosterol (78) |
| erg25 slu2 (*) | Lanosterol (42), 4,4-dimethylzymosterol (58) |
| erg11 erg25 slu1 | Lanosterol (92), 24-methylenelanosterol (8) |
| erg11 erg25 slu2 | Lanosterol (93), 24-methylenelanosterol (7) |
Sterols accumulating in each strain are listed with percentage of each sterol in parentheses. Only the wild-type slu1, slu2, and erg11 erg25 slu1 (slu2) strains were grown aerobically in YPD cultures. Sterol requiring strains (*) were grown in anaerobic jars on solid medium containing exogenous sterol.