Table 1.

Purification of RK expressed from Sf21 cells

Step	Vol, ml	Total protein, mg	Specific activity, nmol Pi/min/mg	Purification, fold	Yield, %
Crude	50.0	128	1.53	1	100
Heparin-Sepharose	12.0	13.5	15.0	9.8	100
Mono Q FPLC	8.0	2.39	69.3	45	86
Mono S FPLC
Fraction 1	8.0	0.210	90.4	59	10
Fraction 2	2.0	0.043	41.9	27	1
Fraction 3	15.0	0.345	96.5	63	17

Starting material was 250 ml of cell culture, 5 × 10⁸ cells. Details are in the text.