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Levels of RpoS (σ38) in KT1008EL cells harboring plasmids with either the PPX1 gene or the control vector during starvation. The plasmids used were pTrcPPX1 or pTrcHisB. Proteins were subjected to Western blotting with anti-σ38 antiserum and visualized with an alkaline phosphatase-conjugated second antibody.
