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Resistance to H2O2 restored by extra copies of the ppk gene
| Vectors* | Genotype | Viability,† % |
|---|---|---|
| Control for PPX1 + control for ppk | Wild type | 100 |
| Control for PPX1 + ppk | ppk+++ | 31 |
| PPX1 + control for ppk | PPX1+++ | 0.5 |
| PPX1 + ppk | PPX1+++ + ppk+++ | 29 |
The plasmids were as follows: control for PPX1 = pLGHisB, control for ppk = pUC18, ppk+++ = pBC29, and PPX1+++ = pLGPPX1.
Stationary-phase cells were exposed to 42 mM H2O2 for 20 min. Viability with no H2O2 exposure is set at 100%.
