Table 3.
Effect of H2O2 and other treatments on pCa50 and Hill coefficient in soleus fibres
| Treatment | ΔpCa50 | ΔnH | n |
|---|---|---|---|
| (1) Repeeted control sequence | −0.002 ± 0.002 | −0.1 ± 0.1 | 28 |
| (2) 5 mm GSH (5 min) at pCa > 9 | −0.004 ± 0.001 | −0.1 ± 0.1 | 3 |
| (3) 10 mm H2O2 at pCa > 9 (5 min) | −0.011 ± 0.008 | −2.1 ± 0.8* | 3 |
| (4) 10 mm H2O2 during activation, | −0.003 ± 0.008 | −1.2 ± 0.2* | 10 |
| subsequent 10 mm DTT (10 min) | −0.003 ± 0.007 | +0.2 ± 0.2 | 7 |
| (5) 10 mm H2O2 during activation, | +0.012 ± 0.004 | −0.9 ± 0.2* | 3 |
| subsequent 5 mm GSH (2 min) | −0.010 ± 0.008 | +0.1 ± 0.1 | 3 |
| (6) 100 μm DTDP (5 min) at pCa > 9, | −0.059 ± 0.027‡ | −1.1 ± 0.5* | 5 |
| subsequent 5 mm GSH (2 min), | +0.001 ± 0.002 | −0.1 ± 0.1 | 5 |
| subsequent 10 mm DTT (10 min) | +0.023 ± 0.011 | +0.3 ± 0.2 | 4 |
| (7) 100 μm DTDP during activation, | +0.353 ± 0.081* | −1.6 ± 0.3* | 5 |
| subsequent 5 mm GSH (2 min), | +0.000 ± 0.002 | 0.0 ± 0.1 | 3 |
| subsequent 10 mm DTT (20 min) | −0.351 ± 0.069† | +1.9 ± 0.2† | 3 |
Mean change (± s.e.m.) in pCa50 and nH resulting from each of the indicated treatments, with n being the number of slow-twitch soleus fibres. Mean (± s.e.m.) values of pCa50 and nH in soleus fibres in the whole study (n = 28) were 6.09 ± 0.02 end 4.3 ± 0.2, respectively.
*
Mean is significantly different from repeated control sequence case (treatment 1; one way ANOVA).
†
Mean change after subsequent treatment (DTT or GSH) is significantly different from zero (Students two-tailed t test).
‡
For treatment 6 indicates significance at P < 0.05 using Student's one-tailed t test. Fibres for treatment 5 are a subset of those used for the initial part of treatment 4.