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. 2002 Nov 15;546(Pt 1):89–100. doi: 10.1113/jphysiol.2002.030510

Table 1.

Increase (Δ) in [Na+]i, [Ca2+]i and pHi induced by fucose-sulphate glycoconjugate (FSG) in the presence and absence of the sperm-activating peptide, speract

Ion ΔFSG ΔSperact + FSG
[Na+]i 31 ± 9 30 ± 6
(mm) (n = 3) (n = 9)
[Ca2+]i 1700 ± 157 1857 ± 220
(nm) (n = 10) (n = 9)
pHi 0.45 ± 0.06 0.42 ± 0.10
(pH units) (n = 10) (n = 9)

Sperm were loaded with Na+-binding benzofuran isophthalate (SBFI AM), fura-2 AM and 2′7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethylester (BCECF AM), as indicated in Methods. the values are the mean ± s.e.m. of the maximum increase (Δ) of at least three experiments.