Table 1.
Increase (Δ) in [Na+]i, [Ca2+]i and pHi induced by fucose-sulphate glycoconjugate (FSG) in the presence and absence of the sperm-activating peptide, speract
Ion | ΔFSG | ΔSperact + FSG |
---|---|---|
[Na+]i | 31 ± 9 | 30 ± 6 |
(mm) | (n = 3) | (n = 9) |
[Ca2+]i | 1700 ± 157 | 1857 ± 220 |
(nm) | (n = 10) | (n = 9) |
pHi | 0.45 ± 0.06 | 0.42 ± 0.10 |
(pH units) | (n = 10) | (n = 9) |
Sperm were loaded with Na+-binding benzofuran isophthalate (SBFI AM), fura-2 AM and 2′7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein, acetoxymethylester (BCECF AM), as indicated in Methods. the values are the mean ± s.e.m. of the maximum increase (Δ) of at least three experiments.