Table 1.
PKC activity in transfected COS-7 cells treated with H2O2
| PKC isoform | H2O2 treatment, min | PKC activity
|
|
|---|---|---|---|
| PSL | % | ||
| α | 0 | 20,300 | 100 |
| α | 5 | 33,500 | 165 |
| α | 10 | 54,600 | 269 |
| βI | 0 | 24,200 | 100 |
| βI | 5 | 35,600 | 147 |
| βI | 10 | 56,900 | 235 |
| γ | 0 | 22,700 | 100 |
| γ | 5 | 46,100 | 203 |
| γ | 10 | 65,500 | 289 |
| δ | 0 | 9,240 | 100 |
| δ | 5 | 49,200 | 532 |
| δ | 10 | 42,000 | 455 |
| ɛ | 0 | 10,400 | 100 |
| ɛ | 5 | 34,000 | 327 |
| ɛ | 10 | 46,800 | 450 |
| ζ | 0 | 14,100 | 100 |
| ζ | 5 | 53,000 | 376 |
| ζ | 10 | 67,100 | 476 |
Phosphorylation of H1 histone by PKC isoforms described in Fig. 2 was quantitated by measuring PSL using a Bio-Imaging Analyzer. The activity of each PKC isoform recovered from the cells without H2O2 treatment was used as 100%.