Table 1.

Primer and probe sequences used for real-time PCR

Gene	Forward primer	Reverse primer
PGC-1α	5′-CAAGCCAAACCAACAACTTTATCTCT-3′	5′-CACACTTAAGGTGCGTTCAATAGTC-3′
NRF-1	5′-GGCACTGTCTCACTTATCCAGGTT-3′	5′-CAGCCACGGCAGAATAATTCA-3′
NRF-2α	5′-AAATTGAGATTGATGGAACAGAGAA-3′	5′-TATGGCCTGGCTTACACATTCA-3′
mtTFA	5′-AGATTCCAAGAAGCTAAGGGTGATT-3′	5′-TTTCAGAGTCAGACAGATTTTTCCA-3,
PPARα	5′-AGCTTTGGCTTTACGGAATACCA-3′	5′-CCACAGGATAAGTCACCGAGGA-3′
Cal B1	5′-CACAGATGGGAATGGAGAAGTAGA-3′	5′-GCAAACCTCAATTTCTGCTCCTT-3′
Cal Aα	5′-GAGGGTGCATCAATTCTTCGA-3′	5′-CAAATCAAAGAATTGTCCATGAATGT-3′
Cal Aβ	5′-AGTGCCACAGTTGAGGCTATTGA-3′	5′-CCAAACCCTTTGCCTCTTCA-3′
CaMKI	5′-TTGCTGGTCCATAGGTGTCATC-3′	5′-AGAGTTTGGCATCATTCTCGTCAT-3′
HKII	5′-TTGTCCGTAACATTCTCATCGATT-3′	5′-TGTCTTGAGCCGCTCTGAGAT-3′
CS	5′-GACTACATCTGGAACACACTCAACTCA-3′	5′-CGCGGATCAGTCTTCCTTAGTAC-3′
HAD	5′-CATAGCGACCAGCACGGAT-3′	5′-GATGGCTTCCACCACCAAGT-3′
Gene	Probe
PGC-1α	5′-AGTCACCAAATGACCCCAAGGGTTCC-3′
NRF-1	5′-ACCACGGTCACCGTTGCCCAA-3′
NRF-2α	5′-AAGCATTGTAGAACAAACCTACGCGCCAG-3′
mtTFA	5′-CCGCAGGAAAAGCTGAAGACTGTAAAGGA-3′
PPARα	5′-TCACGGACACGCTTTCACCAGCTTC-3′
Cal B1	5′-TTTAAAGAATTCATTGAGGGCGTCTCTCAGTTC-3′
Cal Aα	5′-ACAAACAGTGACTGGCGCATCAATATCCA-3′
Cal Aβ	5′-AAGCAATACGAGGATTCTCTCCACCACATAGAA-3′
CaMKI	5′-CCTACATCTTGCTCTGCGGTTACCCTCC-3′
HKII	5′-ACCAAGCGTGGACTGCTCTTCCGA-3′
CS	5′-ACGGGTTGTTCCAGGCTATGGCCA-3′
HAD	5′-CAGCCTCCGTTGTCCACAGCACA-3′

PGC-1 α, peroxisome proliferator activated receptor gamma co-activator 1α; NRF-1, nuclear respiratory factor 1; NRF- 2α a, nuclear respiratory factor 2 α; mtTFA, mitochondrial transcription factor A; PPAR α, peroxisome proliferator activated receptor a; Cal B1, calcineurin subunit B1; Cal A α, calcineurin subunit A α; Cal A β, calcineurin subunit A β; CaMKI, calcium-calmodulin-dependent protein kinase I; HKII, hexokinase II; CS, citrate synthase; HAD, 3-hydroxyacyl-CoA dehydrogenase.