Table 2.
The mRNA content of selected mitochondrial proteins transcription factors and calcium signaling proteins in response to exercise
| Pre | 0′ | 2 h | 6 h | 24 h | ||
|---|---|---|---|---|---|---|
| Transcription factors | ||||||
| NRF−1 | UT | 1 | 1.2 ± 0.1 | 1.0 ± 0.1 | 1.7 ± 0.5 | 0.8 ± 0.1 |
| T | 1.2 ± 0.2 | 1.0 ± 0.1 | 1.2 ± 0.2 | 1.1 ± 0.1 | 1.4 ± 0.4 | |
| NRF−2α | UT | 1 | 1.5 ± 0.4 | 2.0 ± 0.7 | 3.0 ± 0.8 | 2.0 ± 0.6 |
| T | 1.7 ± 0.5 | 2.0 ± 0.6 | 1.9 ± 0.7 | 1.9 ± 0.5 | 1.8 ± 0.4 | |
| mtTFA | UT | 1 | 1.8 ± 0.4 | 1.8 ± 0.5 | 3.4 ± 0.8* | 1.5 ± 0.3 |
| T | 1.6 ± 0.4 | 1.7 ± 0.3 | 2.2 ± 0.4 | 1.9 ± 0.4 | 2.1 ± 0.7 | |
| PPARα | UT | 1 | 1.1 ± 0.1 | 1.0 ± 0.1 | 1.5 ± 0.3 | 1.1 ± 0.2 |
| T | 1.1 ± 0.2 | 0.8 ± 0.1 | 1.8 ± 0.5 | 1.8 ± 0.2* | 0.8 ± 0.2 | |
| Ca2+ signaling proteins | ||||||
| Calcinerium Aα | UT | 1 | 1.3 ± 0.3 | 1.1 ± 0.2 | 2.5 ± 0.6* | 1.3 ± 0.2 |
| T | 1.5 ± 0.3 | 1.0 ± 0.2 | 1.5 ± 0.3 | 1.7 ± 0.3 | 1.4 ± 0.5 | |
| Calcineurin Aβ | UT | 1 | 1.2 ± 0.2 | 0.9 ± 0.1 | 1.6 ± 0.3* | 0.9 ± 0.1 |
| T | 1.0 ± 0.2 | 1.4 ± 0.6 | 1.2 ± 0.4 | 0.9 ± 0.2 | 0.8 ± 0.1 | |
| Calcineurin B1 | UT | 1 | 1.2 ± 0.2 | 1.1 ± 0.2 | 1.2 ± 0.2 | 1.2 ± 0.1 |
| T | 1.4 ± 0.3 | 0.9 ± 0.1 | 1.2 ± 0.2 | 1.0 ± 0.1 | 0.9 ± 0.1 | |
| Calcineurin B1 | UT | 1 | 1.2 ± 0.2 | 1.1 ± 0.2 | 1.2 ± 0.2 | 1.2 ± 0.1 |
| T | 1.4 ± 0.3 | 0.9 ± 0.1 | 1.2 ± 0.2 | 1.0 ± 0.1 | 0.9 ± 0.1 | |
| CaMKI | UT | 1 | 1.5 ± 0.3 | 1.5 ± 0.2 | 2.2 ± 0.7 | 1.3 ± 0.2 |
| T | 2.3 ± 0.3† | 1.8 ± 0.3 | 2.6 ± 0.8 | 2.1 ± 0.5 | 2.5 ± 0.8 | |
| Metabolic enzymes | ||||||
| HKII | UT | 1 | 2.2 ± 0.4 | 4.0 ± 1.7 | 6.2 ± 2.3* | 1.1 ± 0.1 |
| T | 2.2 ± 0.4† | 4.5 ± 1.3† | 4.5 ± 1.3 | 4.6 ± 1.0 | 2.7 ± 0.7 | |
| CS | UT | 1 | 1.1 ± 0.1 | 1.1 ± 0.1 | 1.2 ± 0.2 | 1.2 ± 0.1 |
| T | 1.5 ± 0.1† | 1.4 ± 0.2 | 1.7 ± 0.2† | 1.3 ± 0.1 | 1.4 ± 0.2 | |
| HAD | UT | 1 | 1.2 ± 0.2 | 1.2 ± 0.2 | 1.5 ± 0.4 | 0.8 ± 0.1 |
| T | 1.7 ± 0.2† | 2.0 ± 0.7 | 1.9 ± 0.4 | 1.3 ± 0.3 | 1.4 ± 0.5 | |
The mRNA content of the transcription factors nuclear respiratory factor 1 (NRF−1), Nuclear respiratory factor 2α (NRF−2α), Mitochondrial transcription factor A (mtTFA) and peroxisome proliferators activated receptor α (PPAR α), Selected calcium-signalling proteins calcineurin submit Aα, calcineriun subnit Aβ, Calcineurin suunit B1 and CaMKI (calcium-calmodulin-dependent protein Kinase I), and the matabolioc enzymes hexokinase II (HKII), Citrate synthase (CS) and 3-hydroxyacl-CoA dehydrogenase (HAD) in the hexokinase II (HKII), citrate synthase (CS) and 3-hydroxyacl-CoA dehydrogenase (HAD) in the untrained (UT) and trained (T) leg before (Pre) and immediately after (0′) exercise and at 2,6 and 24 h of recovery from 3 h of two-legged Knee extensor exercise. Values are presented as fold changes relative to Pre, which was set to 1. Values are means ± s.e.m.
Significantly different from Pre in the same leg, P < 0.05
significantly different from untrained leg, P < 0.05.