Table 3.

NRF-1 mRNA content in response to two different exercise protocols

	Pre	0′	15′	1h	2h	4h
One-legged knee extensor exercise	1	1.2 ± 0.2	1.0 ± 0.1	1.2 ± 0.1	1.2 ± 0.2	1.4 ± 0.3
Cycling exercise	1	0.9 ± 02	08 ± 02	0.9 ± 0.3	1.2 ± 0.4	1.3 ± 0.5

The mRNA content of the nuclear respiratory factor 1 in human skeletal muscle in response to exhaustive one-legged knee extensor exercise (70% of 2 min maximum workload, ˜80 min) and to 4 h of cycling at approximately 50% of peak oxygen uptake. Muscle biopsies were obtained from the vastus lateralis muscle before (Pre) and immediately after (0′) exercise and after 15 min (15′), 1h, 2h and 4h of recovery. Values are presented as fold changes relative to Pre, which was set to 1. Values are means 1 S.E.M. These data were obtained from analysis of RNA samples from two studies previously described in detail (Pilegaard et al. 2000). The samples were analysed by conventional RT-PCR using a set of primers (FP 5′-TGGGCGGGAAGACCTTTTGTATG-3′; RP 5′-ATGCTTGCGTCGTCTGGATGGT-3′) different from the set used in the present study.