Table 4.

Characteristics of ¹²⁵I-IL-6 binding to MBH and AP homogenates in the eight experimental groups

		MBH		AP
Sex	Group	Kd (nM)	Bmax (fmol (mg protein)−1)	Kd (nM)	Bmax (fmol (mg protein)−1)
M	Sham ODX	1.3 ± 0.2	34 ± 4	1.3 ± 0.1	31 ± 3
	ODX	1.4 ± 0.2	31 ± 3	1.2 ± 0.1	28 ± 4
	ODX + T	1.4 ± 0.1	32 ± 3	1.4 ± 0.2	33 ± 4
F	Sham OVX	1.2 ± 0.1	29 ± 3	1.2 ± 0.1	32 ± 4
	OVX	1.2 ± 0.2	28 ± 4	1.3 ± 0.2	30 ± 2
	OVX + E2	1.4 ± 0.2	33 ± 4	1.4 ± 0.2	29 ± 3
	OVX + P	1.3 ± 0.2	32 ± 3	1.5 ± 0.2	32 ± 3
	OVX + E2 + P	1.2 ± 0.2	31 ± 4	1.3 ± 0.1	27 ± 4

Six separate binding experiments were performed in each group. For abbreviations, see Tables 1 and 2 and Fig. 4.