Table 5.

Characteristics of ¹²⁵I-TNF-α binding to MBH and AP homogenates in the eight experimental groups

		MBH		AP
Sex	Group	Kd (nM)	Bmax (fmol (mg protein)−1)	Kd (nM)	Bmax (fmol (mg protein)−1)
M	Sham ODX	5.9 ± 0.5	230 ± 21	5.8 ± 0.5	110 ± 16
	ODX	5.7 ± 0.4	512 ± 48*	5.7 ± 0.6	118 ± 13
	ODX + T	5.8 ± 0.6	263 ± 31	5.9 ± 0.5	109 ± 14
F	Sham OVX	6.0 ± 0.5	810 ± 78†	5.6 ± 0.6	113 ± 12
	OVX	5.7 ± 0.6	483 ± 52‡	5.7 ± 0.5	120 ± 15
	OVX + E2	5.8 ± 0.4	836 ± 91†	5.9 ± 0.4	116 ± 11
	OVX + P	6.0 ± 0.5	502 ± 41‡	5.8 ± 0.5	107 ± 13
	OVX + E2 + P	5.9 ± 0.4	825 ± 83†	5.5 ± 0.5	115 ± 12

Six separate binding experiments were performed in each group. For abbreviations, see Tables 1 and 2 and Fig. 4.

^*Statistically significant vs. the other male groups.

^†Statistically significant vs. the OVX, OVX + P and the three male groups.

^‡Statistically significant vs. the sham ODX and ODX + T groups.