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The p90RSK phosphorylation state was determined from vastus lateralis samples (at rest and post-exercise) using Western blot analysis with antibodies specific for p90RSK phosphorylation (Thr573). Equal loading was verified by Ponceau S staining. Data are expressed as arbitrary units (means ±s.e.m.). *P < 0.05 vs. resting. †P < 0.05 vs. age.
