Figure 17. Non-independent sites.

Estimates of k_+1a (A), k_−1b (B) and k_+1b (C) from 1000 simulated fits using simultaneous fit of three concentrations of ACh (10 nm, 30 nm and 100 nm), with a resolution of 10 μs. All three records were fitted in bursts (t_crit= 3.5 ms), using CHS vectors. D, plot of the estimate of k_+1b against that of k_+1a from the same fit (an upper limit of 10¹⁰m⁻¹ s⁻¹ was placed on association rate constants in these fits). The arrows mark the true values of the rate constants.