Figure 2. Boltzmann parameters of motor charge movement determined from capacitance functions during washout of intracellular Cl⁻.

Horizontal bar at top represents extracellular Cl⁻ concentrations ([Cl]_out) during whole-cell recording. Pipette contained either Tris-sulfate or Tris-Hepes solutions (n = 5 for each condition; mean ±s.e.m.). The dominant effect of Cl⁻ washout is a shift in V_pkcm, with small changes in Q_max and z. Error bars are within symbol dimensions for most data points. After removal of extracellular Cl⁻, steady-state levels of parameters show changes that depend upon species of the intracellular substitute anion. Recordings began at the moment of patch rupture at a holding potential of 0 mV.