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. 2003 Jan 17;547(Pt 3):913–929. doi: 10.1113/jphysiol.2002.034611

Figure 6. Effect of PLC inhibition by U-73122 on the TRH effects.

Figure 6

A, TRH-induced r-ERG current suppression is not modified by U-73122. The time course of relative r-ERG current reduction by TRH and E-4031 following a 5 min incubation with 10 μm U-73122 is shown in the middle panel. Filled circles correspond to the current traces shown above. The start of perfusion with U-73122 and the duration of the TRH treatment are marked. Introduction of 5 μm E-4031 into the recording chamber and the averaged magnitude of the current reduction at the end of the 5 min treatment with U-73122 before adding TRH are also indicated on the graph. A comparison of TRH-induced reduction of E-4031-sensitive r-ERG currents for control cells and cells treated with U-73122 is shown in the lower panel. B, the r-ERG current reductions caused by TRH became irreversible in the presence of U-73122. The time course of variations in inward current magnitude in response to an addition of 10 μm U-73122 and the subsequent response to a short application of 100 nm TRH in the continuous presence of the inhibitor are shown in the middle panel. Filled circles correspond to the current traces shown above. A comparison of the magnitude of current recovery upon TRH washout in the absence and presence of U-73122 is shown in the lower panel. Pulse protocols and data evaluation were performed as described in Figs 2 and 3. ***P = 0.0001. Numbers in parentheses indicate the number of experiments.