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. 2003 Feb 7;548(Pt 1):21–29. doi: 10.1113/jphysiol.2002.034918

Figure 5. Distinct effects of PSD-95gfp overexpression on NMDA-mEPSCs in NR2A-flag and NR2B-flag transfected CGCs.

Figure 5

A, comparison of representative traces from CGCs transfected with cDNAs for NR2A-flag vs. NR2A-flag/PSD-95gfp (upper traces) and NR2B-flag vs. NR2B-flag/PSD-95gfp (lower traces) recorded in similar conditions to Fig. 2. Shown on right side of each trace panel are NMDA-mEPSC averages with superimposed double exponential fitting and indication of resulting decay time weighted time constant (τw). Calibration bars are 10 pA and 500 ms (200 ms for averages). B, summary of peak amplitude and τw of NMDA-mEPSCs recorded from CGCs transfected with NR2A-flag vs. NR2A-flag/PSD-95gfp and NR2B-flag vs. NR2B-flag/PSD-95gfp. NMDA-mEPSCs were significantly smaller in cells cotransfected with NR2B-flag/PSD-95gfp than those with NR2A-flag/PSD-95gfp. Both cotransfections shortened the duration of NMDA-mEPSCs. n > 15; *P < 0.05, **P < 0.01 ANOVA.