A, live surface staining of CGCs cotransfected with PSD-95gfp and either NR2A-flag (left) or NR2B-flag (right). Upper panels show PSD-95gfp fluorescence and flag staining. Lower panels (1–6) show a higher magnification of a portion of dendrite (outlined by the box) with PSD-95gfp fluorescence (green, 1 and 4), flag staining (red, 2 and 5) and overlay of PSD-95gfp and flag fluorescence (3 and 6). Calibration bars are 10 μm and 2.5 μm for the insets. B, summary of percentage of colocalization of NR2A-flag or NR2B-flag subunit clusters with PSD-95gfp puncta and percentage of intensity variation of colocalizing clusters vs. mismatching ones. n > 15; *P < 0.05, **P < 0.001 ANOVA.