Table 1.
Compositions of bath and pipette solutions used forelectrophysiological recordings
| Bath | Pipette | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Normal | MVC solution | DVC solution | |||||||||
| Basic | Na+-free | K+ | Li+ | Cs+ | Ca2+ | Ba2+ | Sr2+ | Mg2+ | Mn2+ | ||
| NaCl | 135 | — | — | — | — | — | — | — | — | — | — |
| KC1 | 5 | 5 | 135 | — | — | — | — | — | — | — | — |
| LiCl | — | — | — | 135 | — | — | — | — | — | — | — |
| NMDG | — | — | — | — | — | 125 | 125 | 125 | 125 | 125 | — |
| BaCl2 | — | — | — | — | — | — | 10 | — | — | — | — |
| SrCl2 | — | — | — | — | — | — | — | 10 | — | — | — |
| MnCl2 | — | — | — | — | — | — | — | — | — | 10 | — |
| CsCl | — | 130 | — | — | 135 | — | — | — | — | — | 50 |
| Cs(OH) | — | — | — | — | — | — | — | — | — | — | 90 |
| CaCl2 | 2 | 2 | — | — | — | 10 | — | — | — | — | 3 |
| MgCl2 | 2 | 2 | — | — | — | — | — | — | 10 | — | 2 |
| Glucose | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 5 |
| Hepes | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| EGTA | — | — | — | — | — | — | — | — | — | — | 4 |
| TEA(OH) | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | — |
| Na-ATP | — | — | — | — | — | — | — | — | — | — | 1 |
Concentrations are given in mM and the pH of all solutions was adjusted to 7.3. The osmolarities of the bath and pipette solutions were approximately 340 and 310 mosmol 1−1, respectively. MVC, monovalent cations; DVC, divalent cations.