Figure 6. K252a blocks BDNF-induced reduction of cell surface GABA_A receptor β_2/3 subunit immunoreactivity.

Confocal images of cultured cerebellar granule cells before (A) and following a 60 min exposure to BDNF (100 ng ml⁻¹) (B, C and D). The cultures were fixed and co-stained with antibodies against GABA_A receptor β_2/3 subunits (green fluorescence) and TrkB receptors (red fluorescence). GABA_A receptor β_2/3 subunit immunoreactivity, illustrated in A, is markedly reduced following exposure to BDNF (B). C and D, addition of K252a (200 nm) prevented the BDNF-induced reduction of GABA_A receptor β_2/3 subunit immunoreactivity (C), which persisted in the presence of K252b (200 nm; D). Scale bar (applies to all panels), 50 μm.