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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Dev Biol. 2008 Jan 26;316(1):135–148. doi: 10.1016/j.ydbio.2008.01.017

Figure 8.

Figure 8

Src over-expression mimics the effect of PAO on FM1-43 uptake and cell division. Sea urchin eggs (Lytechinus variegatus) were attached to glass-bottomed chambers and fertilized. At the two-cell stage one of the two blastomeres was injected with cascade-blue dextran (CB-dex, 0.1 mg/ml) alone (panel A), or co-injected with mRNA (0.51 mg/ml) encoding either a constitutive inactive mutant of Src (SrcK277M) or encoding active sea urchin Src (SpSFK1). Next, embryos were incubated for 1 hour at 22°C, and then incubated for an additional 30 minutes in ASW containing 4 μM FM1-43. Embryoes were then washed and imaged by two-photon microscopy. Size bars are 50 μm.