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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Dev Biol. 2008 Jan 26;316(1):135–148. doi: 10.1016/j.ydbio.2008.01.017

Figure 9.

Figure 9

Phorbol ester rescues the agatoxin sensitive form of endocytosis. Either unfertilized eggs (Strongylocentrotus purpuratus, Blue circles) or eggs fertilized in the presence of agatoxin-TK (100 nM, red circles), were incubated for 15 minutes in ASW containing 100 μM tetramethylrhodamine-dextran (to monitor fluid phase uptake) and varying concentrations of PMA. The amount of uptake was normalized to the amount of uptake detected for control eggs (100%) and to fertilized eggs in calcium free ASW (0%). All points are mean ± SD, n=6. Note that the unfertilized eggs had a small negative value. Presumably this is due to a small amount of PAO sensitive uptake in calcium free ASW.