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. 2003 Mar 21;549(Pt 1):3–19. doi: 10.1113/jphysiol.2003.039818

Figure 9. CFTR co-expression confers modest cAMP-sensitivity upon hDRA-mediated Cl−-HCO3− exchange.

Figure 9

A, hDRA-mediated Cl−-HCO3− exchange activity measured by BCECF fluorescence ratio imaging in representative oocytes subjected to sequential Cl− removal and restoration in the absence (○) or presence (•) of 2 mm cAMP plus 1 mm IBMX. B, Cl−-HCO3− exchange activity in representative oocytes expressing CFTR and subjected to sequential Cl− removal and restoration in the absence (▵) or presence (▴) of 2 mm cAMP plus 1 mm IBMX. C, Cl−-HCO3− exchange activity in representative oocytes co-expressing hDRA and CFTR, subjected to sequential Cl− removal and restoration in the absence (▵) or presence (▴) of 2 mm cAMP plus 1 mm IBMX. D, summarized results of Cl−-HCO3− exchange activity for the number of oocytes given in parentheses. Resting pHi was 7.03 ± 0.02 for ‘H2O’ and ‘DRA’ (minus cAMP) groups, and 7.12 ± 0.02 for all other groups. Note that stimulation by cAMP and IBMX is observed only in oocytes co-expressing CFTR and hDRA3. *P < 0.001 compared to water-injected oocytes; **P < 0.001 compared to absence of cAMP/IBMX (adjacent bar).