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. 2003 Mar 28;549(Pt 1):75–92. doi: 10.1113/jphysiol.2002.033472

Figure 1. Identification and morphology of immature Purkinje cells in thin slices.

Figure 1

A, IR-DIC view of Purkinje cells in a cerebellar slice (300 μm thick) of a P4 rat. EGL, external germinal layer. B, confocal image of another P4 Purkinje cell loaded with the fluorescent dye calcein (500 μm) through a whole-cell patch pipette. Overlay of 35 images (taken for every 0.5 μm Z-axis). Calibration bars, 10 μm.