Figure 6. Skew in the current-variance plot by PS-NSFA is expected from the activation kinetics of the Purkinje cell AMPAR model.

Using the kinetic scheme of AMPARs in Purkinje cells (Häusser & Roth, 1997), EPSCs were simulated using four different concentrations of glutamate (0.3 ms, step pulse), as indicated. The single-channel conductance was set at 5 pS, the number of channels was set at 100, and the driving force was −100 mV. A, averages of simulated currents. Upper trace indicates the glutamate pulses. B, current-variance relationships by PS-NSFA (continuous lines) were obtained for an individual set of simulated EPSCs (1000 events each) at indicated peak P_o values. Open circles represent current-variance plots obtained by Sigworth-type NSFA, imposed on the Sigworth-type theoretical relationships (dotted curves). C, current-variance relationships of 4000 pooled events by PS-NSFA.