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The cut-open oocyte voltage clamp technique was used to apply concentrations of NMDG between 0 and 600 mm, and determine the time constants of activation and inactivation. The oocyte was stepped from −90 to +30 mV for 5 s in 0, 100, 300 and 600 mm NMDG (n = 3). Activation and C-type inactivation rates are normalized to enable direct comparison.
