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GMF overexpression up-regulate caspase expression. The mRNA expression levels were determined by quantitative real-time RT-PCR in total RNA isolated from GMF-V or LacZ transfected N18 cells. mRNA levels were estimated by determining ΔCT, the difference between the CT of the caspase of interest and the CT of the endogenous 18S rRNA control gene. * p< 0.05 (GMF-V versus control LacZ).
