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. 2003 May 9;550(Pt 1):3–9. doi: 10.1113/jphysiol.2003.040550

Figure 2. Effect on its protein expression level of antisense oligonucleotide to CD98.

Figure 2

BeWo cells expressing either H2B-GFP or Mit-DsRed2 were mixed, cultured and transfected without or with antisense or mismatch oligonucleotide. CD98 protein levels in the cellular extracts before (control) and after a further 48 h treatment with 100 μM forskolin or with vehicle were analysed as described in Methods. A, Western blot (under reducing conditions). The results presented are from a single representative experiment. B, quantification of CD98 protein levels. The intensity of each band was quantified using an image documentation and analysis system. Data represent the means ± S.D. of three separate experiments, expressed as percentage of control (i.e. values without culture). * Significantly different from control. † Significantly different from values for cells cultured with vehicle alone. ‡ Significantly different from values for cells cultured with forskolin alone. As, antisense oligonucleotide; Mis, mismatch oligonucleotide.