Figure 3. Effect on L-leucine influx of antisense oligonucleotide to CD98.

BeWo cells expressing either H2B-GFP or Mit-DsRed2 were mixed, cultured and transfected without or with antisense or mismatch oligonucleotide. L-Leucine influx was measured over a 3 min period before treatment (control) and after a further 48 h treatment with 100 μM forskolin or with vehicle in a medium containing 2 μM L-[ H]leucine with or without 2 mM 2-aminobicyclo-(2,2,1)-heptane-2-carboxylic acid (BCH) or 2 mM L-leucine in the absence of Na⁺. A, carrier-mediated influx rate defined by subtracting the diffusional component (determined by measuring the influx of 2 μM L-[³H]leucine in the presence of 20 mM unlabelled L-leucine) from the total influx. B, system L-mediated influx rate defined as the difference between the influx in the presence and absence of BCH. Data represent the means ± S.D. of three separate experiments of triplicate assays. * Significantly different from control. † Significantly different from values for cells cultured with vehicle alone. ‡ Significantly different from values for cells cultured with forskolin alone. As, antisense oligonucleotide; Mis, mismatch oligonucleotide.