Figure 7. Gassing of ghost-free buffer and gassing CAII-blocked ghost suspensions reveal identical time courses.
Approach to the physicochemical equilibrium: comparison of the time course of acidification of uncatalysed reactions. A, time course of a ghost-free buffer (PBS: 10 mM, 300 mosmol kg−1, pH: 7.4) solution to which BCECF (free acid) has been added. B, typical time course of acidification with blocked CAII activity through addition of the membrane-permeant ETX (10 μM). The time constants of both assays were not statistically different.