Figure 8. CAII activity in a ghost preparation is not rate limiting under experimental conditions.

A, under the condition of resealing, ghosts were incubated with different amounts of human CAII (0, 200, 400 and 800 μl of 2.5 mg ml⁻¹ CAII). a denotes the fast acidification for the four assays. The statistical error of the initial rates is < 5 %, i.e. CAII load of a ghost preparation does not increase the rate or the ΔpH_i of the fast acidification. B, the rate of acidification after addition of CAII (50 μl) to a ghost suspension was very similar to the rate of acidification calculated for ghosts that had undergone lysis by a 1:6 dilution into hyposmotic (20 mosmol kg⁻¹) PBS.