Figure 13. Comparison of acidification in the intracellular and extracellular compartment in control and inhibition experiments.

Fluorescence inside the cell was measured as usual on BCECF-loaded cells. To measure outside the cells, ghosts were used that were not loaded with dye and added membrane-impermeant BCECF (free acid) to the outer compartment. A, control experiment (vehicle: PBS). B, same as A but with BCECF outside the cells. C, a, HgCl₂ (10 μM) was added prior to DIDS (8 μM); b, DIDS (8 μM) was added prior to HgCl₂ (10 μM). D, same as C a, but with BCECF outside the cells. E, DIDS (10 μM) present in the preparation, incubation 10 min. F, same as E, but with BCECF outside the cells.