Figure 7. The effect of pyruvate on [Ca²⁺]_i transients in the presence of the inhibitor of mitochondrial nucleotide translocation ATR.

A, changes of [Ca²⁺]_i transients after application of 10 mm pyruvate in an intact cell pretreated with 0.3 mm ATR (time in min). B, average normalized data of pyruvate-mediated changes of diastolic [Ca²⁺]_i (○) and [Ca²⁺]_i transient amplitudes (•) in the presence of ATR. Time as in A (w; washout recorded at 4 min after removal of pyruvate). C, examples of [Ca²⁺]_i transients recorded at the times indicated by arrows in A on an expanded time scale. The transients in the presence of ATR (thin line) and ATR + pyruvate (thick line) were normalized and superimposed.