Figure 1.

Immunoblot of an anoxic native gel developed with antibody to γ, illustrating the apoNifH- and MgATP-dependent association of γ₂ with the α₂β₂ apodinitrogenase. FeMo-co insertion reactions containing a fraction enriched in the α₂β₂ apodinitrogenase and γ₂ from DJ54 were subjected to anoxic native PAGE as described. Lanes: 1, reaction including NifH and MgATP; 2, reaction including NifH and excluding MgATP; 3, reaction including apoNifH and MgATP; 4, reaction including apoNifH and excluding MgATP; 5, reaction excluding NifH and MgATP.