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Immunoblot of an anoxic native gel developed with antibody to γ, illustrating γ monomerization upon binding FeMo-co. FeMo-co synthesis reactions were performed as described and subjected to anoxic native PAGE. Lanes: 1, reaction excluding NifH; 2, reaction including NifH; 3, reaction including apoNifH; 4, reaction including NifH and excluding MgATP; 5, reaction including apoNifH and excluding MgATP; 6, purified γ2; 7, purified γ2 plus excess purified FeMo-co.
