Figure 2. Inhibition of SCC reponses to 7,8-benzoquinoline and EBIO.

SCC traces from two Calu-3 monolayers stimulated with EBIO (1-ethyl-2-benzimidazolone; 600 μM, bs) Inhibitors, namely acetazolamide (100 μM, bs) and bumetanide (20 μM, bl), were applied sequentially (A and C), after which the monolayers were thoroughly washed. The monolayers were then re-stimulated with 7,8-benzoquinoline (250 μM, bs) and the inhibitory responses to acetazolamide and bumetanide were repeated (B and D). The ratios of the current removed by bumetanide to that removed by acetazolamide were measured (ratio bumet/acet) and are given in each panel. The shaded area in D represents the current removed by acetazolamide and is equal to 0.52 μequiv.