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Fluorescence in Calu-3 monolayers was measured at 526 nm with excitation alternately at 440 nm and 502 nm. The fluorescence ratio was used to calculate pHi values, as described in the Methods. Addition of 7,8-benzoquinoline (A) caused a fall in pH of 0.3 units. Acetazolamide (100 μM) alone did not affect pHi (B) or prevent pH lowering by 7,8-benzoquinoline.
